TIMP1 antibody

Catalog No. ABIN5690444

This anti-TIMP1 antibody is a Rabbit Polyclonal antibody detecting TIMP1 in WB, ELISA and Neut. Suitable for Human.

Quick Overview for TIMP1 antibody (ABIN5690444)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-TIMP1 Antibody

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTIMP-1 (human Tissue Inhibitor of Metalloproteinases-1). Human TIMP-1 specific antibody was purified by affinity chromatography employing immobilized hTIMP-1 matrix.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human TIMP-1 (3.0 μ,g/mL), a concentration of 5.0 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect hTIMP-1 by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.

Sandwich
To detect hTIMP-1 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human TIMP-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.

Western Blot:
To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: TIMP-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP-1

Background: Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72  kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92  kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/mL) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

Gene ID: 7076

UniProt: P01033